Effects of oral supplementation with plant superoxide dismutase extract on selected redox parameters and an inflammatory marker in a 2,000-m rowing-ergometer test


Journal Title (Medline/Pubmed accepted abbreviation): Int J Sport Nutr Exerc Metab
Year: 2011
Volume: 21
Number:2
Page numbers: 124-134
doi (if applicable): N/A

Summary of Background and Research Design

Background: Physical exercise is known to cause increased formation of reactive oxygen species (ROS), which can potentially overreach antioxidant capacity. The resultant oxidative stress leads to damaged DNA, lipids, proteins, and carbohydrates, and may result in cell dysfunction or death. Enhancing the endogenous antioxidant defense system may reduce oxidative stress and potentially minimize or prevent cell damage. Superoxide dismutase (SOD) catalyzes the reduction of superoxide anions to less reactive hydrogen peroxide and is, therefore, a key component for intracellular antioxidant defense. It is found in many plants; however, its use in dietary supplements has thus far been limited because the enzyme is inactivated upon ingestion. The development of GliSODin is a SOD-rich melon extract that is coupled with a biodegradable wheat-based gliadin biopolymer and is, therefore, delivered efficiently by ingestion.

Hypothesis/purpose of study: The authors hypothesized that supplementation with orally effective vegetable SOD would enhance endogenous antioxidant defense and limit oxidative stress, muscle injury, and inflammatory response during exercise in professional rowers.

Subjects: Study participants (N = 19) were male members of the Polish rowing team. Ten members were assigned to the supplementation group and 9 to the control arm (mean age, 21.3 ± 1.1 yr, 20.3 ± 1.0 yr; mean mass, 89.3 ± 5.3 kg, 86.1 ± 10.2 kg; training, 7.2 ± 1.6 yr, 5.7 ± 1.7 yr, respectively).

Experimental design: Double-blind

Treatments and protocol: Subjects were given 2 capsules of either GliSODin (500 mg) or maltodextrin daily for 6 weeks. Subjects performed a controlled 2,000-m time trial on a rowing ergometer on Day 1 and after 6 weeks of supplementation. The entire study was conducted during a training camp, thus allowing daily monitoring of exercise and limitation of external dietary sources. Blood samples were taken before each 2,000-m trial, 1 minute after exercise, and 24 hours postrecovery, and multiple redox parameters were assessed, including total antioxidant capacity, SOD activity, glutathione peroxidase activity, C-reactive protein, thiobarbituric acid reactive substances (TBARS), lactate dehydrogenase (LDH), and creatine kinase (CK).

Summary of research findings
  • Exercise-induced changes in redox parameters were similar in both supplemented and control groups, with no significant interaction between exercise and supplementation observed.
  • Mean power output and total time did not differ significantly between presupplementation and postsupplementation for either group.
    • Both groups had similarly higher blood lactate levels postexercise after the supplementation period.
  • SOD activity was significantly higher in the supplemented group than in the control group (P = .0037).
    • SOD activity was significantly higher at preexercise, postexercise, and recovery in the supplemented group than in the control group (P < .05).
  • C-reactive protein was significantly lower in the supplemented group than in the control group (P < .001).
  • There were no treatment-related effects on TBARS, CK, or LDH.

Interpretation of findings/Key practice applications

Supplementation with GliSODin did increase SOD activity, but did not alter glutathione peroxidase (another antioxidant enzyme) or TBARS (a marker of oxidative stress). Further, markers of muscle damage (eg, CK, LDH) were not reduced by supplementation. Thus, the physiologic significance of the increase in SOD activity regarding oxidative stress or muscle cell damage is not clear. An additional point is that the authors did not assess the intensity of exercise or the maximum oxygen consumption during the exercise tests, both of which are critical pieces of information. Of note, the observed decrease in C-reactive protein levels after supplementation with GliSODin suggests that melon extract may have anti-inflammatory effects to protect against increased inflammation in the serum of professional rowers. As with the other biomarkers measured, the physiologic significance of a decline in C-reactive protein of the magnitude observed in this study is not known.

One potential limitation of this supplement is that, because of the gliadin coating, it may not be appropriate for athletes with celiac disease or other gluten intolerances.

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